Enzyme kinetics of dl-tetrahydropalmatine and corydalis extract metabolism in rat liver microsomes 延胡索乙素及延胡索提取物在大鼠肝微粒体内代谢动力学研究
Animal experiments with enzyme kinetics and PCR ( polymerase chain reaction) technique were conducted. 用酶动力学和聚合酶链式反应(PCR)对大鼠心肌线粒体呼吸链酶复合物Ⅰ、Ⅳ活性的变化及线粒体DNA的缺失进行测定。
The tartrate resistant acid phosphatase ( TRAP) activities in the medium were measured by enzyme kinetics. 酶动力学法测定培养上清中抗酒石酸酸性磷酸酶(TRAP)的活性;
MtDNA deletions, respiratory chain enzyme complexes and ATP synthesis were divided determined with polymerase chain reaction ( PCR), enzyme kinetics and bioluminescence technique. 心肌线粒体DNA缺失、线粒体呼吸链酶复合体及腺苷三磷酸(ATP)合成分别用聚合酶链反应(PCR)、酶动力学和生物发光技术进行测定。
Studies on Non-Steady State Enzyme Kinetics of Uncompetitive inhibition 反竞争性抑制的非稳态酶动力学布尔函数图解研究
Polymerase chain reaction ( PCR), enzyme kinetics and bioluminescence techniques were used. 用聚合酶链反应(PCR)、酶动力学和生物发光技术进行检测。
Methods The levels of serum enzymes and the content of lipid were detected with enzyme kinetics and chemical coloration end point assay in 92 patients with fatty liver confirmed based on the clinical appearance and B ultrasonography. 方法对92例临床表现符合并经B超明确诊断为脂肪肝的患者采用酶动力学法及化学显色终点法,测定血清酶和脂类含量水平。
A theorem of graph theory in enzyme kinetics 酶动力学计算中的一个图论定理
The enzyme kinetics of 4 SHV types β lactamases from gram negative bacteria were studied spectrophotometrically. 采用分光光度法研究革兰氏阴性菌产生的4种SHV型β-内酰胺酶的动力学。
Based on Phenylacetic acid and Penicillin G dynamics in ion exchange process and immobilized enzyme kinetics, the hydrolysis of Penicillin G was simulated well by computer from the mathematical model developed. 通过确定苯乙酸、青霉素G在离子交换柱中的动力学行为和固定化酶的动力学参数,利用已建立的数学模型,可很好地对青霉素G水解生产6-氨基青霉烷酸过程进行计算机模拟。
Analysis of Non-Steady State Activation Enzyme Kinetics 非稳态酶活化动力学的布尔函数图论分析
Methods: Using cell culture technique, MTT and enzyme kinetics methods. 方法:应用细胞培养技术、噻唑盐比色测定(MTT)和酶动力学方法。
Preliminary Studies of Enzyme Kinetics on Esterase Activity of DNA DNA具酯酶活性的酶动力学初步研究
Methods The contents of serum myocardial enzyme were measured with enzyme kinetics in 187 patients with infective diarrhea. 方法采用酶动力学方法测定187例感染性腹泻患儿血清心肌酶含量。
Studies on Non-Steady State Enzyme Kinetics of Linear Mixed Inhibition When Inhibitor Binds at Two Sites 有两个酶结合位点的线性混合抑制非稳态动力学研究
The dynamic changes of serum CK and CK-BB levels were observed with the enzyme kinetics and agarose gel electrophoresis in the experimental and control groups at sequential time points of 6,12,24,48,72 and 96 hours after injection of bilirubin. 腹腔注射胆红素200mg/kg方法制备胆红素脑病模型,用酶动力法和琼脂糖凝胶电泳法对实验组和对照组注射胆红素后6,12,24,48,72,96h的血清CK及CK-BB水平进行动态观察。
The effects of temperature and relative humidity on berry firmness of grapes were studied, and the prediction model for firmness loss was developed on the basis of enzyme kinetics. 本文研究温度和相对湿度对采后葡萄浆果硬度的影响,并建立了相应的预测模型。
METHODS: Using Enzyme kinetics assay. 方法:酶动力学方法。
The molecular weight of which was determined by SDS-PAGE and the enzyme kinetics parameters of β-lactamas were determined by ultraviolet spectrophotometry. 以SDS-PAGE法测定其分子量及紫外分光光度法测定其酶动力学参数。
Boolean Function Graphic Analysis of Non-Steady State Enzyme Kinetics 非稳态酶动力学的布尔函数图解研究
Characterization and enzyme kinetics of the purified protein demonstrated that the recombinant protein cloned, expressed and purified was human protein kinase CK2 α′ with biological activity. 通过性质鉴定和酶动力学分析证明:克隆、表达和纯化的重组蛋白是有生物学活性的人CK2α′亚基。
In chapter two, a new method for determination of enzyme activity in single cells based on enzyme kinetics with an epi-fluorescence microscopy was developed. 第二章建立了用酶动力学和落射显微荧光成像技术检测单个中性粒细胞内过氧化物酶(PO)活性的新方法。
Some progresses in studies on nonlinear enzyme kinetics 非线性酶动力学某些研究进展
The enzyme kinetics of AmpC β-lactamase in Klebsiella pneumoniae 99-799 and the inhibitory activities of β-lactamase inhibitors were studied by spectrophotometry. 对肺炎克雷伯氏菌99-799株产生的AmpC型β-内酰胺酶利用分光光度法进行了酶动力学及酶抑制剂抑酶效应的研究。
Secondly, we selected two amino acid residues in mature peptide to perform site-directed mutagenesis, and analyzed the structure information and enzyme kinetics. At the same time, we used bioinformatics as supporting research. 其次,我们在NK的成熟肽上,挑选了两个与前导肽有氢键作用的氨基酸残基,分别进行定点突变之后,分析结构信息和酶动力学信息,同时,我们用生物信息学进行辅助研究。
The main work are summarized as follows: The factors affecting the MFC performances were analyzed from enzyme kinetics, chemical reaction theory and mass transfer. 主要工作包括:基于酶动力学理论、化学反应理论和传质学理论分析了MFC工作性能的影响因素。
By substrate depletion approach, the apparent enzyme kinetics parameters michaelis constant ( Km), maximum velocity ( Vmax), and the intrinsic clearance ( CLint) were determined in rat liver microsomes. 利用底物消除法,测定了该类药物在大鼠肝微粒体中的表观酶动力学参数:米氏常数Km、最大反应速率Vmax和固有清除率CLint。
The theoretical basis of MFCs involves engineering thermodynamics, enzyme kinetics and mass transfer, et al. MFC的理论基础包括工程热力学、酶动力学和传质学等。